胶孢炭疽菌C2H2型转录因子CgGcp1的生物学功能

Biological functions of a C2H2 transcription factor CgGcp1 in Colletotrichum gloeosporioides

摘要:

【背景】胶孢炭疽菌(Colletotrichum gloeosporioides)可以寄生于多种植物，侵染方式多样，能够引起严重的农业危害。在胶孢炭疽菌中，CgGcp1是一个C2H2型的转录因子，关于其生物学功能的研究未见报道。【目的】明确CgGcp1的生物学功能，为深入解析该病菌的致病机制奠定一定的理论依据。【方法】构建CgGCP1基因的敲除载体，利用同源重组得到敲除突变体。通过表型分析，包括营养生长、胁迫响应、孢子产生、附着胞形成及致病性分析等，明确该基因的生物学功能。【结果】CgGCP1基因敲除突变体生长速率较野生型减慢，对SDS、刚果红、NaCl和甘油更加敏感，孢子产量显著降低，附着胞的形成率降低且侵入能力减弱，在橡胶叶片上的致病力明显下降。【结论】CgGcp1参与调控胶孢炭疽菌营养生长、细胞壁完整性、分生孢子产生、附着胞形成与侵入和致病性。

Abstract:

[Background] Colletotrichum gloeosporioides, featuring a wide range of hosts and diverse infection methods, causes serious agricultural disasters. The biological functions of CgGcp1 in C. gloeosporioides, a C2H2 transcription factor, have not been reported. [Objective] This study aims to clarify the biological functions of CgGcp1, which is expected to lay a theoretical basis for clarifying the pathogenic mechanism of the fungus. [Methods] The CgGCP1-knockout vector was constructed and the knockout mutant was yielded by homologous recombination. The biological functions of CgGCP1 were identified by pathogenicity analysis and phenotypic analysis, such as the vegetative growth, stress response, conidium production, and appressorium formation. [Results] The growth rate of the CgGCP1-knockout mutant was lower than that of the wild type, and the mutant was more sensitive to SDS, Congo red, NaCl, and glycerol. The conidium yield, appressorium formation rate, and invasion ability were obviously decreased, and its virulence to rubber leaves was also lowered significantly. [Conclusion] CgGcp1 regulates the vegetative growth, cell wall integrity, conidium production, appressorium formation and invasion, and pathogenicity of C. gloeosporioides.