Abstract:Microbiology experiment is the basic course and a core practice course of bioengineering, pharmaceutical engineering, food science and engineering, synthetic biology and other majors. According to the trinity education concept of value shaping, knowledge imparting, and ability training, we set the teaching objectives of microbiology experiment and developed the teaching content system involving value, knowledge, and ability. Further, we improved the teaching contents and methods to keep up with the times, integrated online and offline teaching, standardized the experimental methods, and fused science with education to foster students’ innovative thinking and enthusiasm for research. Additionally, we improved the evaluation system by establishing a comprehensive assessment scheme covering knowledge, ability, and value, and created a microbiology laboratory culture with professional characteristics. The trinity teaching mode of microbiology experiment has been initially established to serve the training of all-round high-quality talents.