马利亚霉菌Mariannaea sp. HJ合成TeNPs及其抗菌和抗氧化性能

Antibacterial and antioxidant properties of tellurium nanoparticles synthesized by Mariannaea sp. HJ

摘要:

【背景】近年来，纳米碲(tellurium nanoparticles，TeNPs)在光电、能源和医学等领域的应用增加。微生物合成TeNPs具有绿色低毒和条件温和等优势，受到了广泛关注，然而目前关于真菌合成TeNPs的研究较少。【目的】探究真菌Mariannaea sp. HJ合成TeNPs的能力及其抗菌、抗氧化性能。【方法】利用真菌Mariannaea sp. HJ合成TeNPs并对其合成条件进行优化，采用扫描电子显微镜、Zeta电位分析及X射线衍射仪对TeNPs进行表征。此外，通过抗菌和抗氧化实验探究TeNPs的应用。【结果】菌株HJ在菌体湿重为1.5 g及TeO₃²⁻浓度为5 mmol/L的条件下还原率最高，合成的TeNPs主要为球形；X射线衍射仪表明TeNPs的结构为六方晶系；FTIR表明羟基、羧基和氨基等官能团可能参与了TeNPs的合成。抗菌实验表明，TeNPs对金黄色葡萄球菌(Staphylococcus aureus)具有良好的抗菌性；抗氧化实验表明，TeNPs对DPPH自由基具有抑制作用，当TeNPs浓度为500 mg/L时抑制率可达80%。【结论】本实验提供了一种TeNPs合成的真菌资源，这为TeNPs的生物合成及应用提供了理论参考。

Abstract:

[Background] In recent years, tellurium nanoparticles (TeNPs) have been widely used in optoelectronics, energy, medicine, and other fields. Featuring mild synthesis conditions and low toxicity, biosynthetic TeNPs have attracted wide attention. However, there are few studies on the synthesis of TeNPs by fungi. [Objective] To investigate the ability of Mariannaea sp. HJ to synthesize TeNPs and the antibacterial and antioxidant properties of the TeNPs. [Methods] We used HJ to synthesize TeNPs and optimize the synthesis conditions. XRD, SEM, and DLS were employed to characterize the yielded TeNPs. In addition, we tested the antibacterial and antioxidant properties of TeNPs through experiments. [Results] The optimal synthesis conditions are as follows: 1.5 g (wet weight) of HJ, and TeO₃²⁻ at 5 mmol/L. The yielded TeNPs were mainly spherical with a hexagonal crystal system (XRD). FTIR showed that functional groups such as hydroxyl, carboxyl, and amino were involved in the synthesis of TeNPs. The TeNPs inhibited Staphylococcus aureus and DPPH (inhibition rate=80%at 500 mg/L). [Conclusion] This study provides a TeNPs-synthesizing fungal strain, which lays a theoretical basis for the biosynthesis and application of TeNPs.