反硝化除磷戈登氏菌属的富集及其菌剂制备

Enrichment and agent preparation of Gordonia for denitrifying phosphorus removal

摘要:

【背景】投加微生物菌剂是强化生物处理效能的重要手段，反硝化是污水脱氮除磷的关键步骤，但目前对于反硝化微生物菌剂相关的研究报道较少。【目的】驯化高效反硝化聚磷菌菌剂，并对系统进行生物强化。【方法】采用两阶段法快速富集反硝化聚磷菌，筛选高效脱氮除磷功能菌株NC1-1并进行鉴定，以NC1-1为菌种来源制备干粉菌剂，研究菌剂强化A²SBR系统污水处理效果。【结果】历经36 d后反硝化聚磷菌富集成功，菌株NC1-1经鉴定属于戈登氏菌属，其脱氮除磷率分别为89.46%和91.68%。麦麸、玉米粉配比为85%:15%、NC1-1投菌量为20 ml、发酵液用量20 ml的条件下成功制得干粉菌剂，干粉菌剂最佳投加量为10%的A²SBR系统总磷(total phosphorus，TP) NO₃⁻-N去除率比未投加菌剂的A²SBR系统提高12.06%和11.52%。【结论】菌剂NC1-1的投加使A²SBR系统的污染物去除效能进一步提高，研究结果为进一步研究反硝化聚磷菌菌剂提供了理论依据。

Abstract:

[Background] Adding microbial inoculum is an important means to enhance the efficiency of biological treatment, and denitrification is a key step in nitrogen and phosphorus removal from wastewater. However, there are few reports on denitrifying microbial agents. [Objective] To acclimate efficient denitrifying phosphorus accumulating organism (DPAO), make the denitrifying agent, and enhance the agent performance. [Methods] The DPAO was rapidly enriched by two-phase method, and strain NC1-1 with high-efficiency nitrogen and phosphorus removal was screened out and identified. The bacterial powder was prepared with NC1-1 strain, and the effect of the powder on wastewater treatment in A²SBR system was studied. [Results] DPAO was enriched successfully after 36 days. Strain NC1-1 was identified as Gordonia, with the nitrogen and phosphorus removal rates of 89.46% and 91.68%, respectively. The bacterial powder was successfully prepared with the wheat bran-to-corn flour ratio of 85%:15%, NC1-1 inoculum volume of 20 mL, and fermentation broth volume of 20 mL. The removal rates of total phosphorus (TP) and NO₃⁻-N in the A²SBR system supplemented with the bacterial powder at a dosage of 10% were 12.06% and 11.52%, respectively, higher than those in the A²SBR system without the bacterial powder. [Conclusion] The adding of NC1-1 enhanced the pollutant removal efficiency of A²SBR system. The research outcome provides a theoretical basis for the further study of DPAO agent.