Abstract:[Background] High wuyiencin-producing genetically engineered strain Streptomyces albulus OoWysR showcases pronounced antifungal effect. [Objective] To identify the active ingredients of S. albulus OoWysR. [Methods] The active ingredients of S. albulus OoWysR were separated and purified by the column chromatography of macroporous resin, ion-exchange resin column chromatography, and high-performance liquid chromatography, respectively. The chemical structures of compounds were identified by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), nuclear magnetic resonance (NMR), and other spectral methods. The biological activity of compounds was determined with the growth rate method. [Results] Four compounds were isolated and identified from S. albulus OoWysR, which were respectively (1) p-hydroxybenzoic acid, (2) pyrrole-2-carboxylic acid, (3) p-hydroxyphenethyl alcohol, and (4) yunnanmycin. Compound 1 showed inhibitory effect on Curvularia lunata, Fulvia fulva, Alternaria alternata, and Bipolaris maydis. Compound 2 exerted certain inhibitory activity against Botrytis cinerea, Sclerotinia sclerotiorum, B. maydis, Valsa ceratosperma, Fusarium graminearum, Pyricularia oryzae, and A. alternata. Compound 3 had specific inhibitory activity against B. cinerea, B. maydis, Botryosphaeria dothidea, and Colletotrichum gloeosporioides. [Conclusion] The active ingredients of S. albulus OoWysR include p-hydroxybenzoic acid, pyrrole-2-carboxylic acid, p-hydroxyphenethyl alcohol, and yunnanmycin, in addition to wuyiencin. This study provides a theoretical basis for further development and utilization of this strain.
