武夷菌素高产基因工程菌株Streptomyces albulus OoWysR活性成分的分离鉴定

Isolation and identification of active ingredients from high wuyiencin-producing genetically engineered strain Streptomyces albulus OoWysR

摘要:

【背景】武夷菌素高产基因工程菌株Streptomyce albulus OoWysR具有明显的抑菌效果。【目的】明确S. albulus OoWysR的活性成分。【方法】采用柱层析法，利用大孔吸附树脂、离子交换树脂和高效液相色谱等对S. albulus OoWysR的活性成分进行分离纯化，经高分辨率电喷雾电离质谱(high-resolution electrospray ionization mass spectrometry，HR-ESI-MS)和核磁共振(nuclear magnetic resonance，NMR)等波谱技术对化合物化学结构进行鉴定，并通过生长速率法测定化合物的生物活性。【结果】从S. albulus OoWysR中分离鉴定出4个化合物，分别为对羟基苯甲酸(1)、吡咯-2-羧酸(2)、对羟基苯乙醇(3)和云南霉素(4)。化合物1对玉米弯孢病菌、番茄叶霉病菌、玉米小斑病菌和烟草赤星病菌具有一定的抑制作用；化合物2对番茄灰霉病菌、大豆菌核病菌、苹果腐烂病菌、玉米小斑病菌、小麦赤霉病菌、稻瘟病菌和烟草赤星病菌具有一定的抑制作用；化合物3对番茄灰霉病菌、苹果轮纹病菌、玉米小斑病菌和黄瓜炭疽病菌具有一定的抑制作用。【结论】S. albulus OoWysR的活性成分除武夷菌素外还包括对羟基苯甲酸、吡咯-2-羧酸、对羟基苯乙醇和云南霉素。本研究结果为进一步开发与利用该菌株提供了理论依据。

Abstract:

[Background] High wuyiencin-producing genetically engineered strain Streptomyces albulus OoWysR showcases pronounced antifungal effect. [Objective] To identify the active ingredients of S. albulus OoWysR. [Methods] The active ingredients of S. albulus OoWysR were separated and purified by the column chromatography of macroporous resin, ion-exchange resin column chromatography, and high-performance liquid chromatography, respectively. The chemical structures of compounds were identified by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), nuclear magnetic resonance (NMR), and other spectral methods. The biological activity of compounds was determined with the growth rate method. [Results] Four compounds were isolated and identified from S. albulus OoWysR, which were respectively (1) p-hydroxybenzoic acid, (2) pyrrole-2-carboxylic acid, (3) p-hydroxyphenethyl alcohol, and (4) yunnanmycin. Compound 1 showed inhibitory effect on Curvularia lunata, Fulvia fulva, Alternaria alternata, and Bipolaris maydis. Compound 2 exerted certain inhibitory activity against Botrytis cinerea, Sclerotinia sclerotiorum, B. maydis, Valsa ceratosperma, Fusarium graminearum, Pyricularia oryzae, and A. alternata. Compound 3 had specific inhibitory activity against B. cinerea, B. maydis, Botryosphaeria dothidea, and Colletotrichum gloeosporioides. [Conclusion] The active ingredients of S. albulus OoWysR include p-hydroxybenzoic acid, pyrrole-2-carboxylic acid, p-hydroxyphenethyl alcohol, and yunnanmycin, in addition to wuyiencin. This study provides a theoretical basis for further development and utilization of this strain.