Abstract:With the continuous enrichment of information technology, the e-education guided by new education concept and combined with online learning platform has become a novel trend of teaching reform in colleges and universities. We introduced science, technology, engineering, art and mathematics (abbreviated to STEAM) education concept to design the overall teaching process and comprehensively optimized the students' learning process by introducing the "online+offline" teaching platform. The original scattered verification and operation experiments were re-integrated into an independent research-based experiment project with multiple tasks as the main line and Blackboard online platform as the auxiliary line. In the novel teaching mode, we taught in a student-centered manner and provided students with more platforms for self-presentation and discussion. Then, we analyzed and evaluated the teaching quality from four aspects:students' performance, knowledge test, after-class feedback, and experimental operation and report. The results indicated that this teaching mode not only improved students' learning quality and subjective initiative in Microbiology Experiments but also cultivated students' ability of problem solving and innovation in practice. This new teaching mode has reference significance for the teaching of experiment courses in other biological disciplines.