Abstract:The basic objectives of specialized courses in higher education are to cultivate application-oriented professionals in line with the needs of social development, and to enhance students' scientific literacy, innovative consciousness, and advanced ability to solve practical problems. Microbial Pharmacology is a major course for undergraduates and postgraduates of Life Sciences, which lays a theoretical foundation for and improves the practical ability of students to engage in scientific exploration, applied research, and industrial production in the future. Based on the concept of application-oriented teaching, this paper discussed the reform of teaching content and system, constructed a diversified practical teaching mode of thematic knowledge-thematic discussion-project-driven teaching-research training-simulation platform-practical learning, and put forward the countermeasures to break the system obstacle and condition restriction. The teaching evaluation results showed that students had positive attitude to the teaching effect and mode of the course, and the teaching objectives of learning for application has been realized. This study provides a reference for strengthening the curriculum construction of applied microbiology.