Medical Microbiology Experiment is a compulsory course in medical universities and colleges, which concerns laboratory biosafety. In order to eliminate hidden dangers over biosafety and avoid problems such as unstable results caused by experiments and atypical biochemical reactions, we have made simulated plastic molds of the typical bacterial cultures and biochemical reaction outcomes of microbiology and have applied them to the experiment teaching. The experiment examination and questionnaire survey results of students were compared between the experiment group and the control group. The results showed that the plastic teaching aids had high simulation degree and met the requirements of observation points in teaching, and students and teachers had high acceptance of the teaching aids. The assessment score of students in the pilot class with application of the teaching aids was slightly higher than that of students in the control class, which indicated the teaching performance as expected. In a word, the simulated teaching aids can guarantee the teaching quality and has a good prospect in the future.