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广陈皮外源真菌的组成及产毒真菌分析
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中国医学科学院医学与健康科技创新工程重大协同创新项目(2017-I2M-1-013)


Toxin-producing fungi and exogenous fungi on Citri reticulatae pericarpium
Author:
  • CHEN Xuyu

    CHEN Xuyu

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, Hainan, China
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  • LIU Xiaomin

    LIU Xiaomin

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, Hainan, China
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  • ZHAO Xiangsheng

    ZHAO Xiangsheng

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, Hainan, China
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  • YANG Meihua

    YANG Meihua

    Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, Hainan, China;National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
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    摘要:

    【背景】广陈皮为药食同源中药材,在高温、高湿且贮存不当的条件下容易发霉,从而产生毒素,严重威胁陈皮的质量安全。【目的】分析广陈皮表面外源真菌的组成及其产生毒素的真菌。【方法】采用平板稀释法分离广陈皮表面外源真菌,利用分生孢子形态特征及DNA序列分析进行真菌鉴定,采用高效液相色谱-三重串联四极杆质谱联用技术对青霉属和曲霉属进行产毒检测。【结果】从广陈皮表面分离外源真菌共132株,鉴定为子囊菌亚门(Ascomycota,98.48%)和毛霉菌亚门(Mucoromycota,1.52%),包括散囊菌纲(Eurotiomycetes,95.45%)、座囊菌纲(Dothideomycetes,3.03%)和毛霉纲(Mucoromycetes,1.52%)。分离活动曲霉属(Aspergillus spp.)菌株共77株,为广陈皮药材外源真菌的优势菌,青霉属(Penicillium spp.)次之。毒素检测筛选出1株产毒真菌,鉴定为黄曲霉(Aspergillusflavus) JXCP1-3。该菌株产生黄曲霉毒素B1 (aflatoxin B1,AFB1),浓度为1.52 ng/mL。【结论】全面分析广陈皮表面的外源真菌及产毒真菌,为广陈皮表面产毒真菌的防控提供依据。

    Abstract:

    [Background] Citri reticulatae pericarpium is a widely used Chinese medicinal material with both medical and edible values.The growing of fungi and the production of mycotoxins by fungi during storage at proper humidity and temperature pose a threat to the quality and safety of Citri reticulatae pericarpium.[Objective] In order to analyze the composition of exogenous fungi and the toxin-producing fungi on Citri reticulatae pericarpium.[Methods] The exogenous fungi were isolated by plate dilution method and identified based on the morphological characteristics and sequence analysis.The toxin-producing fungi were detected by ultra-high performance liquid chromatography coupled to tandem quadrupole mass spectrometry (UPLC-MS/MS).[Results] A total of 132 strains of exogenous fungi were isolated from Citri reticulatae pericarpium.They were identified to belong to Ascomycota(98.48%) and Mucoromycota(1.52%),including Eurotiomycetes(95.45%),Dothideomycetes(3.03%) and Mucoromycetes(1.52%).Seventy-seven strains belonged to Aspergillus spp.,which was dominant on Citri reticulatae pericarpium,followed by Penicillium spp.The strains of Aspergillus spp.and Penicillium spp.were detected by UPLC-MS/MS.One strain of toxin-producing fungi was screened out and identified as A.flavus JXCP1-3,which can produce 1.52 ng/mL aflatoxin B1.[Conclusion] We comprehensively analyzed the exogenous fungi and toxin-producing fungi on the surface of Citri reticulatae pericarpium,which provided a basis for the prevention and control of the toxin-producing fungi.

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陈旭玉,刘小敏,赵祥升,杨美华. 广陈皮外源真菌的组成及产毒真菌分析[J]. 微生物学通报, 2022, 49(3): 1076-1083

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  • 收稿日期:2021-07-23
  • 录用日期:2021-09-13
  • 在线发布日期: 2022-03-07
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