革兰氏阴性细菌蛋白分泌系统研究进展

Progress on the protein secretion system of Gram-negative bacteria

摘要:

革兰氏阴性细菌由于具有复杂的双层膜结构，其蛋白质分泌能力较差。这使得革兰氏阴性细菌的典型菌株——大肠杆菌作为最常用的受体细胞在生物制药工程和其他生物技术产品生产中受到一定的限制。因此，革兰氏阴性细菌蛋白分泌系统的研究具有重要意义。本文详细地归纳了革兰氏阴性细菌已知的蛋白分泌系统，分别从分泌系统的分泌过程、分泌蛋白类别、结构组成和研究技术4个方面做了论述，以期为进一步解析革兰氏阴性细菌蛋白分泌机制提供参考。

Abstract:

Gram-negative bacteria, such as the commonly used Escherichia coli strains, have poor protein secretion capacities due to their intricate double-layer membrane. This shortcoming limits the production of Gram-negative bacteria for specific biopharmaceuticals and other bioproducts. Therefore, the research on the protein secretion system of Gram-negative bacteria is of great significance. This review summarized the knowledge about protein secretion system of Gram-negative bacteria, including the secretion process, the types of secreted proteins, the structural information of the secretion system, and the technologies for protein secretion research. Thus, this review will provide referential information for studying the protein secretion mechanism in Gram-negative bacteria.