适应性实验室进化在工业生产菌株选育中应用的进展

Progress of adaptive laboratory evolution for industrial strain breeding

摘要:

适应性实验室进化是一种在实验室里将微生物置于一定选择压力下，通过长期驯化，筛选获得具有特定表型突变菌株的方法。近年来，该方法通过改进特定进化条件和筛选策略，已广泛应用于筛选具有优良特性的工业生产菌株，如特定表型筛选、底物高效利用、目标产物合成及生长特性优化等工业微生物菌株选育。本文综述了适应性实验室进化技术在工业生产菌株选育中的典型应用实例及研究进展，总结了现存问题及其解决办法，并展望了该技术的应用前景。

Abstract:

Adaptive laboratory evolution is a method of screening mutant strains with specific phenotypes by long-term domestication under certain selection pressure. In recent years, this method has been widely used to screen industrial production strains with excellent characteristics through specific evolutionary conditions and screening strategies, such as specific phenotypic screening, efficient use of substrates, target product synthesis, and growth characteristics optimization. In this review, the typical examples and research progress of adaptive laboratory evolution for the breeding of industrial production strains were summarized, the existing problems and solutions were discussed. The prospect of this technology was also forecasted.