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核桃黑斑病拮抗放线菌WMF106发酵条件优化和抑菌物质稳定性
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陕西省科学院科技计划(2018nk-09,2018nk-01);陕西省自然科学基础研究计划(2020JQ-973);陕西省重点研发计划(2020NY-038)


Optimization of fermentation conditions and stability evaluation of antimicrobial active substance for antagonistic actinomycete WMF106 against walnut blight
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    摘要:

    [背景] 暗蓝色链霉菌WMF106对核桃黑斑病病原菌野油菜黄单胞菌(Xanthomonas campestris pv. campestris)有较好的抑制作用,优化其发酵培养基与发酵条件将为核桃黑斑病生防菌剂的制备与应用提供参考。[目的] 优化核桃黑斑病生防菌株WMF106的发酵条件,测定其抑菌物质稳定性,并通过田间防效测定验证防治效果,为后期规模化发酵以及菌剂的开发奠定基础。[方法] 采用单因素和正交试验优化菌株WMF106的发酵培养基和发酵条件,观察pH、温度、光照、储藏温度对抑菌物质稳定性的影响,了解抑菌物质的极性,测定发酵液的田间防效。[结果] 优化的最佳培养基为酵母浸粉2.0 g/L、燕麦20.0 g/L、NaCl 0.5 g/L、K2HPO4 0.5 g/L、MgSO4·7H2O 0.5 g/L。发酵条件为起始pH 6.0、接种量8%、装液量150 mL/500 mL、发酵时间9 d、发酵温度28 ,具65.33%和61.21%。[结论] 确定了菌株WMF106的最佳发酵培养基和发酵条件,抑菌物质有较好的稳定性及田间防治效果,应用开发前景广阔。

    Abstract:

    [Background] As an important biocontrol actinomyces, Streptomyces caeruleatusWMF106 exhibits good antagonistic effect on the pathogen of walnut blight, Xanthomonas campestris pv. campestris. To promote the preparation and application of antimicrobial agent in the control of the disease, it is necessary to improve the biomass, antimicrobial activity and stability of antimicrobial active substance for strain WMF106. [Objective] The present study aimed to explore an optimal fermentation condition and evaluate the stability and field control effect of antimicrobial active substance for Streptomyces strain WMF106. This study will be benefit for formulating large-scale fermentation procedure and development of bioagent.[Methods] Single factor experiment and orthogonal tests were used to optimize the fermentation medium and conditions. The effects of pH, temperature, light, ultraviolet, storage temperature on the stability of active substances and the polarity nature was determined by the single factor method respectively. To evaluate the results of optimization, the methods of field trials on leaves and fruits were used. [Results] The optimum composition of the fermentation medium was yeast extract 2.0 g/L, octmeal 20.0 g/L, NaCl 0.5 g/L, K2HPO4 0.5 g/L MgSO4· 7H2O 0.5 g/L. The optimized fermentation condition was initial pH 6.0, inoculation amount 8%, medium volume 150 mL/500 mL, fermentation time 9 d, fermentation temperature 28 against the disease that infected leaves and fruits were more than 65.33% and 61.21%, respectively.[Conclusion] The optimal fermentation medium and conditions were obtained. Moreover, it is also concluded that the fermentation broth of strain WMF106 not only possess the great stability, but also have better field control efficacy. Therefore, the strain WMF106 may be considered as potential biocontrol agent against walnut blight.

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瞿佳,孙晓宇,陈锐,门欣,赵玲侠,宁硕瀛. 核桃黑斑病拮抗放线菌WMF106发酵条件优化和抑菌物质稳定性[J]. 微生物学通报, 2022, 49(1): 88-100

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  • 收稿日期:2021-03-18
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  • 录用日期:2021-05-03
  • 在线发布日期: 2021-12-30
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