A virtual simulation experiment teaching platform for a Microbiology Experimental course was established based on the practical application of Microbiology experiment teaching to study the theme of “isolation and identification of Bacillus subtilis in soil”. This paper describes the construction, function and practical application of the platform. By focusing on the role of modern information technology, this platform can be used with traditional classroom teaching to enable effective improvements in teaching and to enhance students’ interest and ability. This study lays a solid foundation for the promotion of teaching reform and innovation of Microbiology Experiments, and the creation of high-level talents.