蔗糖磷酸化酶的研究进展

Research progress on sucrose phosphorylase

摘要:

蔗糖磷酸化酶属于糖苷水解酶13家族，能够催化蔗糖的可逆磷酸解。利用其广泛的底物混杂性，蔗糖磷酸化酶可以将葡萄糖基转移至不同的受体合成熊果苷、甘油葡萄糖苷、低聚糖及多酚化合物的衍生物等产物，这些催化产物可广泛应用于食品、药品、化妆品等行业。随着酶催化技术和蛋白质工程的发展，蔗糖磷酸化酶受到了越来越多的关注，该酶的应用范围也得到了扩大。本文综述了近年来蔗糖磷酸化酶在酶的来源、结构、功能及应用领域等的研究进展，同时讨论了该酶的蛋白质工程改造方法与局限性，并展望了该酶可能的研究方向。

Abstract:

Sucrose phosphorylase (SPase), belonging to the glycoside hydrolase family 13, can catalyze the reversible phosphorolysis of sucrose. Thanks to the broad substrate specificity, SPase can transfer the glucosyl to different acceptors to synthesize arbutin, glycosylglycerol, oligosaccharides, polyphenol derivatives, etc. These products are widely used in food, medicine, cosmetics, and other industries. Owing to the development of enzyme catalysis technology and protein engineering, SPase has received more and more attention, with the application extended. This article reviewed the sources, structures and functions, and application fields of SPase, discussed the protein engineering methods for the enzyme and the limitations, and summarized the future research directions.