Abstract:Microbiological Experiment, which is a compulsory course for biological majors, play very important roles in the cultivation and improvement of basic operation skills and innovation ability of students. In order to solve the problems in the traditional teaching model, a teaching reform based on the characteristics of micro-video of being short, fast and refined is performed. Three kinds of teaching resources are prepared, including basic operation micro-video, courseware micro-video and student experiment micro-video. By combing with other teaching and evaluation methods, the micro-video resources are effective and successfully applied in experimental teaching. Through the teaching model mentioned above, students' interests in learning are aroused, which improved their practical innovation ability and the microbiological experiment teaching quality.