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草菇过氧化氢酶基因(VvCAT1)异源表达及耐温度胁迫功能分析
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上海市科技兴农项目(2020-02-08-00-12-F01479)


Expression of catalase gene (VvCAT1) from Volvariella volvacea in Escherichia coli and its temperature tolerance
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    摘要:

    [背景] 过氧化氢酶(catalase,CAT)参与真菌的生长发育,逆境胁迫时保护真菌免受氧化损伤。[目的] 实现草菇过氧化氢酶基因(VvCAT1)的异源表达,分析VvCAT1耐温度胁迫的功能。[方法] 克隆VvCAT1,构建过表达载体pBAR GPE1/VvCAT1,转化到大肠杆菌(Escherichia coli)菌株Stbl3中,异源表达草菇过氧化氢酶。测定温度胁迫后重组菌(pBAR GPE1/VvCAT1/Stbl3)与对照菌(pBAR GPE1/Stbl3)的过氧化氢酶活性和生长情况,验证VvCAT1的功能。[结果] 重组菌的CAT酶活性显著提高,生长情况显著优于对照菌。[结论] VvCAT1的导入及表达显著提高了大肠杆菌Stbl3的耐温度胁迫功能。

    Abstract:

    [Background] Catalase (CAT) is involved in the growth and development of fungi and protects them from oxidative damage in the event of stress. [Objective] The VvCAT1 gene from Volvariella volvacea was expressed in Escherichia coli Stbl3 and its temperature tolerance was characterized. [Methods] VvCAT1 was cloned to construct a recombinant plasmid pBAR GPE1/VvCAT1 which was then transformed into Stbl3 to yield a recombinant strain expressing catalase. Catalase activity and growth of the recombinant strain (pBAR GPE1/VvCAT1/Stbl3) and the control strain (pBAR GPE1/Stbl3) were measured to verify the temperature tolerance. [Results] The recombinant strain exhibited stronger heat and cold tolerance than the control with increased catalase activity and better growth. [Conclusion] The transgenosis and expression of VvCAT1 significantly improved the heat and cold tolerance of E. coli Stbl3.

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杨焕玲,常婷婷,赵妍,游华芳,查磊,李正鹏,仝宗军,陈明杰. 草菇过氧化氢酶基因(VvCAT1)异源表达及耐温度胁迫功能分析[J]. 微生物学通报, 2021, 48(11): 4054-4060

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  • 收稿日期:2021-02-24
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  • 录用日期:2021-04-29
  • 在线发布日期: 2021-11-11
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