Abstract:Nitrite-dependent anaerobic methane oxidation (N-DAMO) is a key link that couples the nitrogen cycle and the carbon cycle. It is mediated by Candidatus Methylomirabilis oxyfera. The discovery of the N-DAMO process has important implications for the study of the global carbon and nitrogen cycling. Through literature review, this article firstly summarized the influencing factors of N-DAMO reaction at home and abroad, N-DAMO reaction under different natural ecosystems. Then, it elaborated the functional structure, physiological and biochemical characteristics of N-DAMO bacteria, the enrichment and culture optimization of N-DAMO bacteria, and research techniques for N-DAMO reaction. Finally, the application research of N-DAMO was discussed. This article not only helps to reveal the coupling mechanism of global carbon and nitrogen cycles, but also provides a theoretical basis for N-DAMO reaction coupled with other anaerobic biological treatment processes to be applied to wastewater carbon and nitrogen removal.