高通量测序分析甘肃高寒草原瑞香狼毒根内生菌群落结构特征研究

High throughput sequencing analysis of Stellera chamaejasme L. root endophytic microorganisms in Gansu alpine grassland

摘要:

[背景] 瑞香狼毒是我国天然草地退化的标志性植物之一，在入侵的过程中其根内微生物发挥着重要的作用，探究瑞香狼毒根内微生物群落对有益微生物资源的利用及生物防控瑞香狼毒具有重要意义。[目的] 探究甘肃省祁连山东麓不同高寒草原毒性杂草瑞香狼毒根部内生真菌和细菌的组成、多样性等群落结构特征。[方法] 通过Illumina MiSeq高通量测序技术对甘肃祁连山东麓不同高寒草原的瑞香狼毒根部内生菌组成及多样性进行分析。[结果] 不同高寒草原瑞香狼毒根内子囊菌门（Ascomycota）在真菌中占优势地位；蓝细菌（Cyanobacteria）、变形菌门（Proteobacteria）、放线菌门（Actinobacteria）在根内生细菌中属于优势类群。G4样点的真菌和细菌OTU （Operational Taxonomic Unit）数量和Shannon多样性指数均高于其他4个采样点。主坐标分析（Principal Coordinate Analysis，PCoA）表明，不同高寒草原瑞香狼毒根内生菌群落之间存在一定的差异。One-Way ANOVA分析结合Pearson相关性分析显示，不同样地瑞香狼毒真菌群落结构在属水平上相关性较小，而细菌在属水平存在一定的相关性。[结论] 本研究为进一步开发利用瑞香狼毒内生真菌和细菌资源、阐明瑞香狼毒内生菌群的群落结构和生态功能提供理论参考。

关键词:内生微生物;瑞香狼毒;高通量测序技术

Abstract:

[Background] Stellera chamaejasme L. is one of the landmark plants of natural grassland degradation in China. The root endophytic microorganisms play an important role in the process of invasion. It is of great significance to explore the root endophytic microbial community to the utilization beneficial microbial resources, and to prevention and control of the Stellera chamaejasme L. [Objective] The purpose of this study was to explore the endophytic fungi and bacteria composition and diversity in the roots of Stellera chamaejasme L., a poisonous weed in different alpine grasslands at the eastern foot of Qilian Mountains in Gansu province. [Methods] The composition and diversity of endophytic fungi and bacteria in the roots of Stellera chamaejasme L. in different alpine grasslands at the eastern foot of Qilian Mountains in Gansu province were analyzed by Illumina MiSeq high-throughput sequencing technique. [Results] The results showed that Ascomycota in different alpine steppe was dominant in fungi in the roots of Stellera chamaejasme L. from different alpine grasslands. Cyanobacteria, Proteobacteria and Actinobacteria belong to the dominant groups in root endophytic bacteria. The operational taxonomic unit (OTU) number and Shannon diversity index of fungi and bacteria in G4 sampling site were higher than those in the other four sampling sites. Principal coordinate analysis (PCoA) showed that there were some differences among endophytic fungi and bacteria communities in the roots of Stellera chamaejasme L. in different alpine grasslands. One-Way ANOVA analysis and Pearson correlation analysis showed that the community structure of Stellera chamaejasme L. fungi had little correlation at the genus level, while the bacteria had a certain correlation at the genus level.[Conclusion] The purpose of this study is to further develop and utilize the endophytic fungi and bacterial resources and provide theoretical reference for elucidating the community structure and ecological function of endophytic flora of Stellera chamaejasme L.

Key words:endophytic microorganisms;Stellera chamaejasme L.;high-throughput sequencing technology