Abstract:The construction and application of online courses has become an important measure in the reform of teaching informatization in colleges and universities at this stage. Effective application of online course resources and the design and practice of blended teaching are the key points in the construction of first-class undergraduate courses. Relying on the online excellent course of microbiology (Shaoxing city, Zhejiang province), the blended teaching based on online course was tried in the science education major of grade 16, 17 and 18. The three rounds of teaching practice showed:the pattern could enhance the students' interest and participation in learning, promote the students' deep learning, and promote the improvement of students' comprehensive ability and quality. The microbiology course has formed a brand at the college and school, and has been widely welcomed and praised by students.