Abstract:Honey bees are very important pollinators to agriculture. Gut microbiota is closely related to honey bee health, and affected by many external factors. Herein, the influences on gut microbiota diversity of Apis mellifera ligustica caused by diseases, antibiotics used for disease treatment, and pesticides were analyzed. Furthermore, the researches on application of honey bee gut microbiota as probiotics was summarized. The prospect of research on honey bee and its microbiota is suggested.