Abstract:[Background] Cyanophages, are viruses infecting cyanobacteria and a potential factor to control the growth and elimination of cyanobacteria blooms, which are of great significance to the regulation of cyanobacteria community structure. A large number of studies previously reported have revealed the high diversity of cyanophages in marine and freshwater environments, however, the knowledge of cyanophages in wetlands is scant. [Objective] It is necessary to clarify the genetic diversity of cyanophages gene g20 in Napahai plateau wetland in China, and provide a theoretical basis for further research on microbial resources and ecological functions of plateau wetlands. [Methods] The water samples were collected during the rainy season. The capsid protein gene g20 as marker gene was amplified by PCR with specific primers Cps1/Cps8 in this study, and 26 different effective sequences of gene g20 were obtained. The principal coordinates analysis and phylogenetic analysis for gene g20 sequences from above and other ecological environment were performed. [Results] The phytophagy sequences in Napahai plateau wetland were more closely related to rice field sequences compared with other marine and freshwater phytophagy sequences. However, some of the sequences were clustered separately, they may be unique type of cyanophages in Napahai plateau wetland. [Conclusion] It shows that cyanophages is abundant and show unique in Napahai plateau wetland.
