微生物多糖合成关键基因挖掘的研究进展

Summary of progress in mining crucial genes involved in microbial polysaccharides synthesis

摘要:

随着分子生物学技术的快速发展，功能基因的挖掘在微生物高产多糖合成关键途径研究中变得越来越重要，不断发展的基因挖掘方法和基因组分析工具推进了研究的深入进行。本文主要综述了近年来报道的微生物多糖生物合成途径和多糖合成途径中的关键酶，以及利用多种技术手段和分析软件工具对多糖合成关键基因进行挖掘和验证的相关研究，为微生物多糖合成关键基因的验证以及微生物高产多糖菌株的制备提供参考。

关键词:微生物多糖，多糖合成途径，基因挖掘，功能验证

Abstract:

Profiting from the rapid development of molecular biology technology, functional genomics plays an increasingly important role in analyzing microbial polysaccharide synthesis pathways. Various gene mining methods and genomics analysis tools facilitate the study of genes responsible for microbial polysaccharides synthesis. Here, we summarize the recent studies in biosynthetic pathway of versatile microbial polysaccharide biosynthesis and the key enzymes involved, the techniques and software package useful for gene mining and function verification. This review will provide a reliable technical reference for crucial genes verification in microbial polysaccharide synthesis and feasible solutions in strain engineering with elevating production titer.

Key words:microbial polysaccharides, polysaccharide synthesis pathway, gene mining, function verification