Abstract:[Background] Bacillus amyloliquefaciens strain BS-3 is an endophytic bacterium from healthy rubber tree roots, and shows strong antimicrobial activity in vitro. The strain has the potential to be used as a biological fungicide. [Objective] To analyze the genome information of B. amyloliquefaciens BS-3 strain, to help further study the antimicrobial mechanism and secondary metabolites biosynthetic gene clusters of BS-3 strain. [Methods] The genome was sequenced using BGISEQ II platform and PacBio RS III platform, then analyzed using relevant software for genome assembly, gene prediction and functional annotation, prediction of secondary metabolite synthetic gene clusters. [Results] The genome is 3 870 130 bp with average GC content of 46.88%, which might code the potential 4 161 genes; and the genome of strain BS-3 had 92 tRNA; 28 rRNA; 10 sRNA; 122 tandem repeats, 98 minisatellites DNA and 2 microsatellites DNA. There were up to 2 875, 2 620, 1 885, 4 040 and 3 328 potential genes annotated with COG, GO, KEGG, NR and Swiss-Prot databases respectively. At the same time, 10 secondary metabolite biosynthetic gene clusters were also predicted, which might code the surfactin, fengycin, bacillaene, bacillibactin and other antibacterial substances. The sequencing data from this article are available in the GenBank database (accession No. CP060384). [Conclusion] This study provides basic data to analyze the internal causes of the biocontrol activity effect of strains BS-3 at the genomic level, and provides reference information for further understanding the secondary metabolic synthesis pathway of B. amyloliquefaciens. It is of great significance to the further study BS-3 strains.
