节杆菌(Arthrobacter sp.)降解阿魏酸的效能

Degradation of ferulic acid by Arthrobacter sp.

摘要:

【背景】蓄积在土壤中的阿魏酸类化感自毒物质对农作物生长产生危害，利用有益微生物分解该类物质是一项有效的治理措施。【目的】从自然界土壤分离获得能高效降解阿魏酸的菌株，并评估典型环境因子对降解效能的影响，以期为该菌在阿魏酸类自毒物质降解领域中的应用提供理论依据。【方法】采用一次性投加高浓度化合物的驯化方法分离筛选得到能有效降解阿魏酸的菌株，采用16S rRNA基因序列分析进行菌株鉴定，采用高效液相色谱法跟踪不同条件下该菌株对阿魏酸的降解情况。【结果】分离筛选获得的菌株为节杆菌属细菌(Arthrobacter sp.)，命名为J6；在≤1.20%盐度(即12 g/L NaCl)、pH 5.0?9.0、20?40 °C、有/无葡萄糖和有/无酵母粉的条件下，菌株J6均能高效降解阿魏酸。【结论】菌株J6在阿魏酸类自毒物质的生物修复领域具有较高的应用潜能。

Abstract:

[Background] Ferulic acid and its analogues, accumulated in soil, are harmful to crop growth. It is an effective solution to decompose these substances by microorganisms. [Objective] A strain that could degrade ferulic acid efficiently was isolated from the soil. The ferulic acid degradation efficiency of the strain was evaluated for further application of the strain. [Methods] The strain was screened by the special culture containing high concentration of ferulic acid. The partial 16S rRNA gene sequences were used to identify the strain. The ferulic acid degradation characters of the strain were detected by HPLC. [Results] The strain was Arthrobacter sp. and named J6. High degradation efficiency was obtained at ≤1.20% saline stress (i.e., 12 g/L NaCl), pH 5.0?9.0, temperature at 20?40 °C, with/without glucose and with/without yeast. [Conclusion] strain J6 had good application potential in bioremediation of ferulic acid.