单细胞拉曼技术在病原微生物检测中的研究进展

Detection of pathogenic microorganism by single-cell raman spectroscopy: a review

摘要:

单细胞拉曼技术是基于拉曼光谱分析原理实现非培养、无标签、快速、高效、低成本揭示物质本质的新方法。近年来，单细胞拉曼技术也开始在病原微生物检测领域崭露头角。本文结合单细胞拉曼技术基本原理这一理论基础，阐述该技术在病原微生物鉴定、药物敏感性检测中的研究进展及最新技术方向，并探讨其在临床实验室应用的可行性，为未来病原微生物检测技术提供新的方向。

Abstract:

Single-cell raman spectroscopy (SCRS) is an emerging technology that reveals the intrinsic biochemical profiles of individual cells. SCRS is non-destructive, label-free, and independent of cultivation, thus it is deemed as a rapid and efficient technology with low cost. Based on the principles of raman spectroscopy, herein, we summarize research progress and the latest technical direction of this technology in pathogen identification and drug susceptibility test, and then discuss the application feasibility in a clinical laboratory to provide a new option for the detection, identification and antimicrobial susceptibility testing of pathogenic microorganisms in the future.