远海50个站位沉积物中潜在木质素降解菌的分离与鉴定

Isolation and identification of potential lignin-degrading bacteria in sediments from 50 stations of the Pelagic Ocean

摘要:

【背景】微生物在海洋木质素的降解过程中发挥了至关重要的作用，然而来源海洋环境的木质素降解菌的相关研究报道却很少。【目的】从远海沉积物环境分离潜在的木质素降解菌，为木质素的可再生化学物质转化提供菌种资源。【方法】利用以木质素为唯一碳源的培养基，对50个远海沉积物样品中的木质素降解菌进行富集培养与纯化，并利用含苯胺蓝的脱色培养基筛选潜在木质素降解菌，继而通过16S rRNA基因测序与序列比对初步确定潜在木质素降解菌的分类地位。【结果】从50个沉积物样品中共分离获得菌株283株，其中潜在木质素降解菌263株，它们隶属于α-变形杆菌纲(Alphapreobacteria)、γ-变形杆菌纲(Gamaproteobacteria)、芽孢杆菌纲(Bacilli)、放线杆菌纲(Actinobacteria)和黄杆菌纲(Flavobacteriia)中的32个属。【结论】远海沉积物环境中蕴含着丰富的木质素降解菌，为海洋生物资源的开发利用提供了新的参考。

关键词:远海，木质素降解菌，鉴定

Abstract:

[Background] Microorganisms play a vital role in the degradation process of marine lignin, but there are few reports about the lignin-degrading bacteria that originate from the marine environment. [Objective] To isolate potential lignin-degrading bacteria from sediments of Pelagic Ocean, and to provide strain resources for the conversion of renewable chemical substances of lignin. [Methods] Enrichment and purification of lignin-degrading bacteria in 50 sediment samples from the Pelagic Ocean by using the medium with alkaline lignin as the sole carbon source, and then screen for potential lignin-degrading bacteria by using the decolorizing medium. The taxonomic status of potential lignin-degrading bacteria preliminarily determined by 16S rRNA gene sequencing and sequence identity comparison. [Results] A total of 283 strains were isolated from 50 sediment samples, including 263 potential lignin-degrading strains, which belonged to 32 genera in five class (Alphapreobacteria, Gamaproteobacteria, Bacilli, Actinobacteria and Flavobacteriia). [Conclusion] The sediment environment in the Pelagic Ocean is rich in lignin-degrading bacteria, which provided new references for the development and utilization of marine biological resources.

Key words:Pelagic Ocean, lignin-degrading bacteria, identification