Abstract:Online learning platforms such as MOOC are the result of education informatization, and the breakthrough in traditional teaching combined with MOOC is one of the hot topics of recent teaching reform. Microbiology Engineering course is a compulsory course for biotechnology and bioengineering majors in colleges. It is a strong applied and practical course. In order to deepen students? understanding of theoretical knowledge and cultivate students? scientific thinking, we have introduced an open teaching model based on MOOC in the Microbiological Engineering experiment course. The results showed that open teaching under the background of MOOC had a good effect in enhancing learning interest, training creative thinking, enhancing scientific research literacy, and cultivating teamwork ability of students.