枯草芽胞杆菌基因组删减对异源酶表达影响的研究进展

Advance in the effect of reduction of the Bacillus subtilis genome on the expression of heterologous enzymes

摘要:

枯草芽胞杆菌作为一种遗传背景清晰、基因编辑成熟的革兰氏阳性菌，是多种重要工业酶的生产宿主。随着转录组、蛋白质组、代谢组等多组学测序和分析技术的发展，通过合理设计简化枯草芽胞杆菌基因组，减少细胞内冗余的调控和代谢网络，使得细胞更精简且便于控制，展现出了枯草芽胞杆菌作为异源酶表达宿主细胞的应用潜力。本文简要综述了枯草芽胞杆菌基因组删减的研究进展，归纳了必需基因的确定方法，重点介绍了枯草芽胞杆菌通过删减基因组提升异源酶表达的研究进展及删减策略，充分展示了枯草芽胞杆菌基因组删减在构建异源酶表达底盘细胞中的重要作用。

Abstract:

Bacillus subtilis as a Gram-positive bacterium, which has clear genetic background and mature gene editing, and it was also considered as the important host of industrial enzymes. With the development of multi-omics sequencing technologies including transcriptome, proteome and metabolome, the rational design could simplify the Bacillus subtilis genome, reduce redundant regulatory and metabolic networks. It makes Bacillus subtilis more streamlined and easily to control. Furthermore, Bacillus subtilis as a heterologous enzyme expression host has been emerged the potential applications. This article briefly reviewed the advance in the effect Bacillus subtilis genome reduction, summarized the essential genes and the methods to identify them in Bacillus subtilis, highlighted the research progress and deletion strategies of Bacillus subtilis to enhance heterologous enzymes by deleting the genome, and demonstrated the important role of genome reduction in the construction of heterologous enzyme expression on the cellular chassis.