Abstract:[Background] Soil microorganisms are very sensitive to the changes of the microenvironment where they live. Bird island, as a lakeside wetland, is sensitive to climate change. However, there are few studies on soil microorganisms at bird island in Qinghai lake. [Objective] In order to explore the changes of soil microbial community characteristics in the bird island of Qinghai lake after climate warming. [Methods] Open-top chamber simulation was used to understand the community structure and diversity changes of soil bacteria and fungi after temperature rise through high-throughput sequencing method. [Results] The increase in temperature did not change the dominant microbial flora of soil microorganisms in bird island of Qinghai lake. The dominant bacterial flora was Proteobacteria and Acidobacteria; the dominant fungi were Ascomycota. Temperature increase changed the soil microbial community structure, significantly increased the relative abundance of Bacteroidetes, Cyanobacteria, Patescibacteria and Cystobasidiomycetes, and significantly reduced the relative abundance of Leotiomycetes. The diversity index of the soil microbial community has changed. After the temperature rises, the ACE index and Chao1 index of the microorganisms decrease, and the Simpson index of bacteria and Shannon index of fungi decrease. [Conclusion] Soil microorganisms respond significantly to temperature rise, and the community structure and diversity of microorganisms have changed since temperature rise.