林可霉素生产中三级种子罐的发酵工艺优化

Optimization of fermentation process of the third-stage seed fermenter in lincomycin production

摘要:

【背景】林可霉素是一种在临床应用上占有重要地位的林可酰胺类抗生素，关于调控发酵生产中三级种子罐相关参数优化林可霉素发酵工艺的研究较少。【目的】优化林可霉素发酵工艺，提高林可霉素发酵效价及市场竞争力。【方法】对林可霉素生产中三级种子罐的培养基和接种量及三级种子移种菌龄进行优化。【结果】在三级种子罐培养基中葡萄糖、淀粉、玉米浆、黄豆饼粉和硫酸铵浓度分别为64.0、5.0、15.0、14.5和3.5 g/L，三级种子罐接种量为25%及三级种子移种菌龄为60 h的优化条件下，林可霉素四级发酵效价高达7 883 U/mL，比优化前效价提高了10%。【结论】对林可霉素生产中三级种子罐相关参数进行调控，初步优化了林可霉素发酵工艺，提高了发酵效价，为优化林可霉素发酵工艺提供了新思路。

Abstract:

[Background] Lincomycin is a lincomamide antibiotic that plays an important role in clinical application, few studies can be found on regulating the parameters of third-stage seed fermenter to optimize the lincomycin fermentation process. [Objective] The fermentation process of lincomycin was optimized in order to improve the fermentation titer and market competitiveness. [Methods] The medium and inoculation amount of the third-stage seed fermenter and the transplanting age of third-stage seeds in lincomycin production were optimized. [Results] The concentrations of glucose, starch, corn pulp, soybean meal and ammonium sulfate in the third-stage seed fermenter’s medium were 64.0, 5.0, 15.0, 14.5 and 3.5 g/L, respectively. The inoculation amount of third-stage seed fermenter and the transplanting age of third-stage seeds were 25% and 60 h, respectively. Under the optimized conditions, the fourth-stage fermenter’s titer of lincomycin was up to 7 883 U/mL, which was 10% higher than that of the before optimization. [Conclusion] Regulating the parameters of third-stage seed fermenter in lincomycin production, the fermentation process was preliminary optimized and the fermentation titer was improved. It provides a new idea for optimization of lincomycin fermentation process.