Abstract:Mind maps have been used as a mind tool in various fields. This plan focused on variations from hand-painted to software drawing; from designated topic to free-choice topics; from independent work, pair work to group work; thus gradually applied mind map to Microbiological courses teaching and learning. The observation and investigation methods were used for evaluation by teacher and students. Mind maps could be helpful in students’ understanding, and knowledge structure construction; thinking process and communication. Finally, we paid attention to the value of mind maps in connecting biological branches. Mind map is a tool for graphical thinking, teamwork, and knowledge management that is worth practicing.