新疆棉花黄萎病棉株与健康棉株内生古菌定量及其时空变化规律

Quantitative analysis of endophytic archaea and its spatiotemporal variation in verticillium wilt and healthy cotton plants from xinjiang

摘要:

【背景】现今棉花黄萎病严重阻碍棉花的稳定高产，妨碍棉花产业的发展。在生物防治中内生菌潜力巨大，但关于内生古菌含量在棉花黄萎病棉株的变化规律鲜有报道。【目的】研究不同生育期以及不同植棉地区黄萎病棉株和健康棉株内生古菌的分类学信息和数量变化规律。【方法】采用Miseq高通量测序和taqMan探针实时荧光定量PCR技术，对新疆棉花黄萎病棉株、健康棉株不同生育期和不同典型生态区的内生古菌进行定量分析。【结果】内生古菌在新疆各采样地和不同生育期的棉花黄萎病棉株、健康棉株内的群落组成相似。在不同生育期，新疆黄萎病、健康棉株内生古菌数量呈先增加后减少然后趋于平缓的趋势，蕾期达到最高值。在不同地区，新疆黄萎病棉株内生古菌数量在北疆地区最高，其次是东疆地区，最后是南疆地区。健康棉株则是南疆地区最高，东疆次之，北疆最低。【结论】新疆黄萎病棉株、健康棉株内生古菌数量在不同的生育期以及不同空间存在较大差异，整体变化趋势显著，可为后续研究提供相关理论支撑。

Abstract:

[Background] At present, verticillium wilt of cotton seriously hinders the stable and high yield of cotton and hinders the development of cotton industry. Endophytic bacteria have great potential in biological control, but the variation of endophytic archaea in verticillium wilt of cotton is rarely reported. [Objective] In order to provide theoretical support for the following studies, the quantitative changes of endophytic archaea in cotton plants with verticillium wilt and healthy plants in different growth periods and different planting areas were studied. [Methods] Using MiSeq high-throughput sequencing and TaqMan probe real-time fluorescence quantitative PCR technology, the endophytic archaea of verticillium wilt and healthy strains in Xinjiang cotton were quantitatively analyzed at different growth stages and in different typical ecological regions. [Results] The community composition of endophytic archaea was similar to that of verticillium wilt and healthy cotton plants at different sampling sites and different growth stages in Xinjiang. At different growth stages, the number of endophytic archaea in verticillium wilt and healthy cotton plants in Xinjiang increased first, then decreased, then tended to be flat, and reached the highest value in bud stage. In different regions, the number of endophytic archaea of verticillium wilt cotton in Xinjiang was the highest in the northern region, followed by the eastern region and the southern region. Health plant is the highest in the southern region, followed by the eastern region, the lowest in the northern region. [Conclusion] The quantity of endophytic archaea in cotton plants with verticillium wilt and healthy plants in Xinjiang was significantly different in different growth periods and different spaces, and the overall variation trend was significant.