萘普生降解菌群的驯化及其降解效能

Acclimatization and biodegrading efficiency of the naproxen-degrading microbial community

摘要:

【背景】萘普生是一种被广泛使用的非甾体抗炎药，治疗人类疾病的同时对环境产生一定的消极影响，甚至危害到人类的生存环境。【目的】利用微生物降解萘普生类污染物是一种价格低廉且行之有效的方法。【方法】以萘普生为唯一碳源，培养驯化高效的萘普生降解菌群；利用高通量测序技术解析萘普生降解菌群的微生物群落变化，鉴定萘普生降解菌群种类；通过GC-MS分析萘普生降解菌群的降解途径。【结果】获得了以Rhodanobacter为主的萘普生高效降解菌群，确定了萘普生降解菌群的最佳降解条件为：30 °C、pH 7.0、摇床转速150 r/min、接种量10%，萘普生降解率达60.58%，并预测出萘普生降解菌群的降解途径。【结论】获得了高效的萘普生降解菌群，明晰了降解机理和降解途径，不仅丰富了微生物资源种类，更为微生物的工程应用奠定了理论基础。

Abstract:

[Background] Naproxen is a widely used drug which is non-steroidal and anti-inflammatory. It has certainly negative impact on the environment while treating human diseases. What’s more, it maybe even endangers the human living environment. [Objective] The way of degrading naproxen pollutants through microorganisms is inexpensive and effective. [Methods] In this paper, naproxen was used as the sole carbon source to cultivate domesticated and highly effective naproxen-degrading bacteria; The high-throughput sequencing technology was used to analyze the microbial community changes of naproxen-degrading bacteria and to identify the types of naproxen-degrading microbial community; Analysis of degrading pathway of naproxen-degrading microbial community by GC-MS. [Results] Finally, a highly effective naproxen-degrading microbial community was mainly Rhodanobacter, and the optimal growth degradation condition of naproxen-degrading microbial community was determined to be 30 °C, pH 7.0, 150 r/min, 10% inoculation amount, with naproxen degrading rate reached 60.58%, and predicting the degradation pathway of naproxen-degrading microbial community. [Conclusion] Obtaining a high-efficiency naproxen- degrading microbial community, and clarifying the degradation mechanism and degradation pathway. The research not only enriches the types of microbial resources, but also lays the theoretical foundation for microbial engineering applications.