Abstract:In 1984, Harvard University professor Eric Mazur founded peer-instruction (PI) in physics teaching practice, PI guides students to explore actively, learn from each other and obtain good teaching effect. In the 21st century, with the rapid development of information technology and life science, the experimental teaching of life science is also moving towards the network platform. In the blended teaching of Microbiology Experiment, the PI method is applied. In the explanation of experimental principle, operation technology, analysis of experimental results and mutual evaluation of homework, the PI method keeps the learning process at the state of teaching to others all the time, that is, the retention rate of knowledge and skills is stable at 90% after two weeks. Comparing the classes with PI method and classes without PI method, the classes that implemented the peer teaching method have significantly improved in the statistics of various achievements. Therefore, PI method in experimental teaching is an effective teaching method worthy of further research and promotion.