Abstract:The main target of Microbiological Experiment is not only to improve the students’ experimental skills, but also cultivate students’ ability to analyze and solve problems. Although traditional teaching model is helpful for students to grasp the experimental skills in a short period of time, it also restricts their subjective initiative. Combined the traditional teaching model with personalized teaching method, students are totally involved in the experiments. They began to know how to find a reference, design an experiment, analyze the data and write a scientific paper. This manuscript introduced how to apply personalized teaching model in Microbiological Experiments.