Abstract:In the first-class undergraduate talent training, the “golden class” are strictly required to be built around the standard, namely high-level, innovative and challenge. On basis of the Microbial Physiology course, the national microbiology teaching team of Nankai University reorganized the teaching content and produced the online course Microbial Physiology. The course team also used online course to carry out a flipped classroom and online- offline teaching. We add difficulty to the course content, and students need more time to study and think after class; the course content also adds frontier and timeliness; the teaching form fully reflects the advanced teaching mode through IT-assisted teaching tools. Teachers strictly control all aspects of classroom teaching, using the new techniques and methods such as Rain classroom, Blue ink, and Wisdom tree to assist teaching, including students’ attendance, class activation, quizzes, effective evaluation, summarize and other teaching activities; through interactive and inquiry-based teaching, we organize students to conduct high-quality discussions in classroom, and cultivate students’ ability to actively learn, think and express; adopt diversified homework design system and course assessment methods to develop students’ ability to think independently and apply what they have learned. Microbial Physiology has been successfully launched on the Chinese University MOOC platform. Through the construction and application of the Microbial Physiology online course, it was successfully transformed from the “knowledge transfer type” course to the “capacity training type” course.