In view of the demand of health profession for medical biotechnology professionals in higher vocational colleges, the course of “Learning field” of Microbial Technology should be set up to meet the needs of enterprise employment groups. Develop and design the “Learning situation” teaching unit oriented by the working process in the production or service practice of the enterprise; Carry out the project teaching of integration of theory and practice and the interactive teaching reform of cloud class. We have adopted diversified assessment forms, guided by products or project achievements, to provide students with opportunities to perceive and experience the work process. Through the long-term intensive training of “Learning situation” project teaching, students have accumulated comprehensive skills and quality to adapt to the future post.