Abstract:In view of the current situation and characteristics of the students majoring in biotechnology, case teaching reform is explored in microbiology class. In the Microbiology teaching, students sought for cases after class and shared them in class, teachers introduced some practical cases in the theories teaching, and the classical scientific research cases were deconstructed by teachers and students. The purposes of stimulating students’ learning initiative, promoting knowledge internalization and transformation and cultivating innovative thinking in scientific research, were attained. The practice teaching proves that case teaching can effectively stimulate study enthusiasm and improve comprehensive quality of students.