参豉对气虚血瘀模型大鼠肠道微生物及其代谢多样性的影响

Effect of Shenchi on intestinal microbiota and their metabolism diversity in rats with qi deficiency and blood stasis

摘要:

【背景】参豉为人参与大豆采用自淡豆豉中分离获得的优势益生菌株共同发酵而成。已证明对气虚血瘀模型大鼠血液指标具有明显的调节作用，但对肠道微生态作用尚不明确。【目的】以气虚血瘀模型大鼠为材料，探讨参豉对肠道微生态是否具有调节作用。【方法】采用长期“力竭游泳+饥饿”方法建立大鼠气虚血瘀模型，造模同时分别灌胃给药参豉高、中、低剂量(每日6、3、1.5 g/kg体重)及补阳还五汤剂60 d后，分析肠道6种常驻菌群数量变化；并采用Biolog-ECO自动微生物鉴定系统研究大鼠肠道微生物的代谢情况。【结果】参豉能够促进气虚血瘀模型大鼠肠道中有益菌脆弱拟杆菌、乳酸菌及双歧杆菌的增殖，调节肠杆菌及肠球菌数量使其趋于正常水平，并抑制有害菌产气荚膜梭菌的增殖。Biolog结果显示参豉高剂量组AWCD值与空白组接近；培养48 h时，模型组Shannon指数、Shannon均匀度、Simpson指数以及Mclntosh指数均显著高于空白组，差异显著(P<0.05或P<0.01)，而参豉高剂量组Shannon指数、Shannon均匀度、Simpson指数与空白组相接近，差异无统计学意义(P>0.05)，与模型组相比差异极显著(P<0.01)；聚类分析与主成分分析显示参豉各剂量组与空白组、模型组、补阳还五汤组差异明显，可能与不同剂量参豉对气虚血瘀模型大鼠肠道微生态的调节作用相关。【结论】参豉对气虚血瘀模型大鼠肠道菌群具有明显的改善作用。

Abstract:

[Background] Shenchi was obtained through co-fermentation with the soybean (Glycine max (L.) Merr.), Ginseng (Panax Ginseng C.A.Mey.), and the fermentation probiotics (Bacillus subtilis subsp. subtilis), that was obtianed from the Sojae Semen Praepartaum in our laborary. It has obvious regulation effect on blood index of rats with qi deficiency and blood stasis, and the effect on intestinal micro-ecology is still unclear. [Objective] In order to investigate the effection, that the intestinal micro-ecology for Shenchi on of rats with qi deficiency and blood stasis. [Methods] The rat model of qi deficiency and blood stasis was constructed by the method, that was “exhaustive swimming+restricted diet” with a long-term. Animal models were treated with the high, medium and low doses of Shenchi and Buyang Huanwu Decoction by intragastric administration for 60 days. After 60 days’ treatment, analyzed change in the number of six resident bacteria; and the carbon sources metabolism was determined by Biolog-ECO microbial identification system. [Results] The results showed that the Shenchi could promote the proliferation of beneficial bacteria including Bacteroides fragilis, Lactobacillus and Bifidobacterium in the intestinal flora of rats with qi deficiency and blood stasis, the shenchi could regulate the number of Enterobacter and Enterococci to normal levels, and inhibit proliferation of Clostridium perfringens. Biolog results showed the AWCD value of the high dose group, which was similar to the normal group. At 48 h cultivation period, the value of Shannon index, Shannon evenness, Simpson index and Mclntosh index were significantly higher in the model group than in the normal group, (P<0.05 or P<0.01). The Shannon index, Shannon evenness, and Simpson index of the high-dose group were similar to the normal group (P>0.05), and the values were significantly difference compared with the model group (P<0.01). Cluster analysis and principal component analysis showed that different dose groups of Shenchi were distinct from the normal group, model group and Buyang Huanwu Decoction group, which may be related to the regulation of intestinal microbes in rats with qi deficiency and blood stasis. [Conclusion] Shenchi had a significant improvement on the intestinal flora of rats with qi deficiency and blood stasis.