环糊精葡萄糖基转移酶高效异源表达研究进展

Advance in high-level heterologous expression of cyclodextrin glycosyltransferase

摘要:

环糊精葡萄糖基转移酶(cyclodextrin glycosyltransferase，CGTase)酶法合成环糊精是目前生产环糊精的主要方法。本文介绍了用于生产环糊精葡萄糖基转移酶的几种工程菌株：大肠杆菌、枯草芽孢杆菌以及毕赤酵母，其中大肠杆菌是目前应用最广泛的用于表达CGTase的表达系统。除此之外，本文还总结了高效表达环糊精葡萄糖基转移酶的有效策略：选择合适的表达载体、启动子以及信号肽，以及密码子优化和分子伴侣共表达，以期为在相关CGTase研究领域开展研究提供参考。

关键词:环糊精葡萄糖基转移酶，大肠杆菌，枯草芽孢杆菌，酵母，异源表达，策略

Abstract:

The cyclodextrin glycosyltransferases (CGTases) are enzymes that used to synthesize cyclodextrin, and this method of production of cyclodextrin is mostly used at present. we described several engineering strains which were used to produce CGTase including Escherichia coli, Bacillus subtilis and Pichia pastoris, and the expression system of E. coli is one of the most widely used. In addition, we also summarized the effective strategies for high efficient expression of CGTase including appropriate expression vector, promoter and signal peptide, codon optimization, co-expression with molecular chaperone. The purpose of this review is to provide a reference for relevant research of CGTase.

Key words:Cyclodextrin glycosyltransferase (CGTase), Escherichia coli, Bacillus subtilis, Yeast, Heterologous expression, Strategy