宏基因组学在微生物抗生素抗性基因检测中的应用

Application of metagenomics in the detection of microbial antibiotic resistance genes

摘要:

抗生素广泛应用于人类和动物疾病的治疗等过程中。不合理利用和滥用抗生素导致耐药细菌、抗性基因的产生和传播。宏基因组学能够分析不同环境中抗生素抗性基因的多样性，并且完善目前已有的或构建新的宏基因组文库，从而为将来进行基因比对提供有力的参考。本文将综述宏基因组学在人类、动物和环境中微生物抗生素抗性基因检测的应用，以期为未来评估抗性基因风险和解决抗生素耐药性问题提供技术支持。

Abstract:

Antibiotics are widely used in the treatment of human and animal diseases. Unreasonable use and abuse of antibiotics lead to the production and spread of resistant bacteria and resistance genes. Metagenomics can analyze the diversity of antibiotic resistance genes in different environments, and improve the existing or construct new metagenomic libraries, which will provide powerful references for future gene comparison. This article will review the application of metagenomics in the detection of microbial antibiotic resistance genes in humans, animals and the environment, and provide technical support for future assessment of resistance gene risks and addressing antibiotic resistance.