甾类化合物微生物转化与分解代谢机制研究进展

Advances in microbial transformation and catabolic mechanism of steroid compounds

摘要:

甾类化合物具有重要的生理医药作用，市场需求巨大。甾类化合物及其关键甾类药物通过微生物转化制备工艺较化学合成法具有区域立体选择性、减少合成步骤、缩短生产周期、提高收率以及生态友好等优点逐步被应用，然而甾类物质微生物分解代谢机制还有待进一步深入探索研究并确定。本文从甾类化合物结构种类与主要来源、生理功能、微生物转化与分解代谢机制的研究等方面进行了归纳，着重解析甾类化合物分解代谢过程关键酶系及其分子作用机制，为甾药化合物生产菌种改造与工程菌构建，以及微生物转化工业化生产工艺的开发提供参考。

Abstract:

Steroid compounds plays an important role in physiological and pharmaceutical effects and the market demand is very huge. The microbial transformation process of steroid compounds and their key steroids is gradually applied since its application offers a number of advantages over chemical synthesis as follows: regio-stereoselectivity, reducing the synthesis procedures, shortening the production cycle, improving yield, more eco-friendly process and so on. However, the mechanism of microbial catabolism of steroids is still to be further explored and determined. The essay comprehensively analyzes the structure types and main sources, physiological functions, microbial transformation and catabolic mechanisms of steroid compounds and focuses on the key enzymes and their molecular mechanisms of metabolic process of steroids, which can provide a reference for construction of engineering strains and the development of industrial production technology for microbial transformation of steroids.