The gastrointestinal flora plays an important role in animal health and production performance. The application of probiotics in livestock and poultry can regulate intestinal micro-ecological balance, regulate fat metabolism, improve feed utilization and promote the formation of flavor substances. It can improve meat quality, included carcass quality of livestock and poultry. This paper reviews the mechanisms what regulates gastrointestinal microecology by different probiotics, and improves the meat quality of livestock and poultry by improving the structure of intestinal flora. It can reference for further research and development of probiotic feeds targeting gastrointestinal flora.