几种真姬菇菌种保藏方法的保藏效果对比

Effect comparison of several preservation methods for strains of Hypsizygus marmoreus

摘要:

【背景】菌种保藏方法与保藏效果是菌种质量的前提和保证。随着真姬菇Hypsizygus marmoreus栽培规模和产量的逐年递增，选择最佳的菌种保藏方法也越来越迫切。【目的】对真姬菇菌种的常见保藏方法及保藏效果进行分析和研究，寻找简便、高效、廉价的真姬菇菌种保藏方法。【方法】通过对比菌丝生长率、菌丝脱氢酶活性以及菌丝对色度培养基的脱色率等指标参数来评价保藏方法的保藏效果。【结果】通过对三大类27种真姬菇菌种保藏方法3个月保藏效果的对比分析发现，三大类菌种保藏方法中保藏效果最好的是水溶液保藏法，其中以0.1% PEG6000水溶液保藏效果最佳；其次为木屑保藏法，其中以盐水浸泡过的杨木木屑保藏效果最好；斜面保藏法效果最差。正常保藏实验的效果好于加速保藏实验。【结论】与当前真姬菇菌种保藏的常规方法相对比，本实验所采用的菌种保藏方法大大拓宽了菌种保藏方法的种类与范围，提高了菌种保藏的效果。

Abstract:

[Background] The preservation method and its effect are the premise and guarantee for the quality of strains. It is more and more urgent to select the best preservation method for strains of Hypsizygus marmoreus with the increasing of cultured scale and yield year by year. [Objective] Some common preservation methods and their effects were analyzed and studied in order to find a simple, efficient and inexpensive preservation method for strains of Hypsizygus marmoreus. [Methods] The preservation effect of each preservation method was evaluated by comparing the growth rate of mycelial, dehydrogenase activity of mycelial and the decolorization rate of mycelium to LBL medium. [Results] By comparing and analyzing the three-month preservation effects of three kinds, 27 different preservation methods of Hypsizygus marmoreus strains, it was found that the best preservation method was water solution preservation, among which 0.1% PEG6000 water solution was the best; the second was sawdust preservation method, among which the saline-soaked poplar sawdust was the best; The slant preservation method was the third. The effect of normal preservation experiment was better than that of accelerated preservation experiment. [Conclusion] Compared with the conventional preservation methods for strains of Hypsizygus marmoreus, the preservation methods adopted in this experiment greatly broadened the types and scope of the preservation methods, and improved the preservation effects of strains.