来自土壤的金孢属四个新记录种

Four new soil-inhabiting Chrysosporium records

摘要:

【背景】金孢属是一类重要的嗜角蛋白真菌资源。【目的】对我国甘肃省和云南省土壤中的金孢属资源进行调查。【方法】采集不同环境中的土壤样品，利用鸡毛和头发对土壤样品中的这类资源进行钓饵，分离目标菌株；基于形态特征和ITS rDNA序列进行系统发育分析相结合的方法进行菌株的鉴定。【结果】从土壤中分离发现5个金孢属菌株，其中菌株H5.11在形态上与节状金孢模式菌株的形态特征非常相近，而且系统发育中与GenBank上报道的该种序列以较高的支持率聚在一个亚分支；H10.10菌株的形态特征与乔治金孢模式标本的形态非常吻合，培养基上均呈现红色，同时系统发育分析结果表明该菌株与GenBank上报道的序列较好的聚在一个亚分支中；EB8803M和EB8801M两个菌株在形态特征上与水生金孢模式标本的描述非常吻合，系统发育分析结果显示它们与网上该种序列能较好地聚在一个亚分支中；O1菌株在形态特征上与轮带金孢模式标本的描述非常吻合，其系统发育表明它与GenBank已报道菌株序列能较好聚在一起。因此将它们鉴定为节状金孢Chrysosporium articulatum、乔治金孢C. georgiae、水生金孢C. submersum和轮带金孢C. zonatum。【结论】这4个种均为中国新记录种。

Abstract:

[Background] Chrysosporium was a kind of important keratinophilic fungi. [Objective] The Chrysosporium resources in soil samples from Gansu and Yunnan provinces were investigated. [Methods] Different soil samples were collected, and cultivated with the chicken feather and hairs by baiting technique, then the target strains were isolated and identified by the morphological characteristics and phylogeny of ITS rDNA sequences. [Results] There were five target strains of Chrysosporium obtained, strain H5.11 was very similar to the original description of C. articulatum in morphology and clustered into a subclade with the sequence of C. articulatum from GenBank in phylogenetic tree; Strain H10.10 was consistent with C. georgiae in morphology, both of them appeared red colony, and it was closely related to the type sequence of C. georgiae in phylogeny; Strains EB8803M and EB8801M had similar morphological characters to C. submersum, and it clustered together with sequences of C. submersum from GenBank in phylogenetic tree; Strain O1 was similar to the original description of C. zonatum in morphology and it also clustered together with the sequence of C. zonatum from GenBank. Therefore, these strains were identified to four species, C. articulatum, C. georgiae, C. submersum and C. zonatum. [Conclusion] These four species are new to China.