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基于代谢组的厌氧氨氧化菌群对温度的响应机制
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国家自然科学基金(51878008)


Metabolomics insight into the response mechanism of anammox consortia to temperature
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    摘要:

    【背景】在环境工程领域中,大多数耐冷菌从活性污泥中分离得到。了解活性污泥菌群对低温的响应有助于耐冷菌的驯化培养。【目的】以厌氧氨氧化污泥菌群作为研究对象,研究温度对厌氧氨氧化菌群代谢通路与代谢产物的影响,以期初步阐释厌氧氨氧化菌群低温响应机理。【方法】在25 °C与35 °C条件下驯化培养厌氧氨氧化污泥,研究温度对反应器脱氮效能、菌群活性与生长以及群落结构的影响,通过代谢组学比较两个温度下厌氧氨氧化菌群代谢物丰度以及代谢通路活性。【结果】虽然低温导致厌氧氨氧化菌群CO2固定、TCA循环与丙酮酸代谢的下调,进而导致氮去除活性以及增长速率显著下降,但菌群RNA合成水平、腐胺与信号分子合成上调,从而通过转录调控、调控膜脂组成与改变膜结构的方式,调控菌群代谢以适应低温环境。【结论】从分子机理的角度探究了厌氧氨氧化污泥菌群适应低温环境的生理机制,首次阐释了腐胺与信号分子在污泥菌群适应低温过程中的重要作用。

    Abstract:

    [Background] Most cold-tolerant bacteria are isolated from activated sludge. Understanding the response of activated sludge consortia to low temperatures contributes to the domestication of cold-tolerant bacteria. [Objective] Anammox consortia was studied as the research object to study the effects of decreasing temperature on metabolic pathways and metabolites of anammox consortia, in order to explain the response mechanism of anammox consortia to decreased temperature. [Methods] The anammox sludge was domesticated at 25 °C and 35 °C to study the effects of temperature on the nitrogen removal performance of the reactors, bacterial activity and biomass yield, and community structure. Metabolite abundance and metabolic pathway activity of anammox consortia were compared under two temperatures by metabolomics. [Results] Under decreased temperature, although CO2 fixation, TCA cycle and pyruvate metabolism of anammox consortia were down-regulated, which led to a significant decrease in nitrogen removal activity and biomass yield. However, RNA synthesis level, and synthesis of putrescine and signal molecules were up-regulated, thereby regulating the metabolism of the consortia to adapt to the low temperature through transcriptional regulation, regulation of membrane lipid composition and changes in membrane structure. [Conclusion] The physiological mechanism of anammox consortia adapting to low temperature was explored from the perspective of molecular mechanism, and the important role of putrescine and signal molecules in the adaptation of sludge consortia to low temperature was explained for the first time.

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霍唐燃,潘珏君,刘思彤. 基于代谢组的厌氧氨氧化菌群对温度的响应机制[J]. 微生物学通报, 2019, 46(8): 1936-1945

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  • 在线发布日期: 2019-07-25
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