培养基中添加海藻糖对大球盖菇、斑玉蕈菌丝生长的影响

Effect of adding trehalose to culture medium on the growth of Stropharia rugosoannulata and Hypsizigus marmoreus

摘要:

【背景】大球盖菇和斑玉蕈是食药兼用且具有开发潜力的珍稀食用菌，培养基对菌丝生长及子实体发育具有重要作用，优化培养基显得尤为重要。【目的】筛选出最适合培养大球盖菇、斑玉蕈的新型培养基。【方法】使用添加海藻糖的新型培养基，对不同培养基培养的大球盖菇及斑玉蕈菌株的菌丝生长状况、生长速度和生物量及纤维素酶、漆酶活性进行测定与分析。【结果】相较于PDA培养基，添加海藻糖的培养基能够提高菌丝生长速度、增加生物量，海藻糖添加的比例对纤维素酶和漆酶的影响较大，对大球盖菇及斑玉蕈菌丝的生长产生显著的促进作用，但不会改变其蛋白质的组成。【结论】大球盖菇最适合选用PTA-5培养基，斑玉蕈的最佳培养基是PDTA培养基。

Abstract:

[Background] Stropharia rugosoannulata and Hypsizigus marmoreus are rare edible and medicinal fungi with great development potential. The medium plays an important role in mycelial growth and fruit body development. Optimizing the medium of the two mushroom is particularly important. [Objective] Screen out the new medium more suitable for S. rugosoannulata and H. marmoreus cultivation. [Methods] Using new media containing trehalose for the cultivation of S. rugosoannulata and H. marmoreus. The mycelial growth status, growth rate and biomass, cellulase and laccase activities were measured and analyzed in different culture media. [Results] Trehalose-added media promoted the growth rate of mycelia and increased the biomass compared with PDA medium. The proportion of trehalose content had a great effect on cellulase and laccase activity. Trehalose-added media could significantly promote the growth of hyphae, but the protein composition did not change. [Conclusion] PTA-5 was more suitable for the cultivation of S. rugosoannulata mycelia; PDTA was more suitable for the cultivation of H. marmoreus mycelia.