细菌3-酮脂酰ACP还原酶研究进展

Research advances on bacterial 3-oxoacyl-acyl carrier protein reductase

摘要:

3-酮脂酰ACP还原酶(FabG)在细菌中广泛存在并且十分保守，已经发现的所有FabG及其同系物都具有类似的催化活性中心序列，隶属于短链醇脱氢酶/还原酶(SDRs)超家族成员。它是II型脂肪酸合成反应中的关键酶，将3-酮脂酰ACP还原为3-羟脂酰ACP多以NADPH作为辅酶。从搜集的文献来看，国内外针对不同细菌中3-酮脂酰ACP还原酶同系物的研究报道体现了其多样性的特点。但是，近年来，该方面的专题综述十分少见。本文主要对3-酮脂酰ACP还原酶的结构特征、在脂肪酸合成以及其他方面的生物学功能，以及以该酶为作用靶点的抑菌剂等方面进行概述，以期为将来3-酮脂酰ACP还原酶的深入研究提供理论参考。

Abstract:

3-oxoacyl-acyl carrier protein reductase (FabG) are ubiquitously expressed enzymes that catalyse the reduction of acyl carrier protein (ACP) linked thioesters within the bacterial type II fatty acid synthesis (FASII) pathway, it reduce 3-oxoacyl-ACP to 3-hydroxyacyl-ACP, usually using NADPH as co-factor. FabG belongs to a broad superfamily of short-chain alcohol dehydrogenase/reductase or short-chain dehydrogenases/oxidoreductases (SDRs). The research reports of 3-oxoacyl-ACP reductase homologues in different bacteria show the characteristics of their diversity. However, in recent years, the summaries in this area have been very rare. In order to provide a theoretical reference for further research on 3-oxoacyl-ACP reductase, this review mainly summarizes the protein structures, function on fatty acid synthesis pathways and other biological functions of 3-oxoacyl-ACP reductase, antibiotics that target this enzyme.