基于蛋白质组芯片的结核分枝杆菌系统生物学研究进展

Advances in system biology of Mycobacterium tuberculosis based on proteome microarray

摘要:

近些年全球结核病疫情愈发严重，耐药性结核病使其雪上加霜。一个重要原因是结核病新药的匮乏以及结核分枝杆菌相关基础研究的不足。因此迫切需要开发新的技术以促进结核病系统生物学基础研究，并在此基础上研究新机制，发现新靶标，开发新药物。结核分枝杆菌功能蛋白质组芯片的出现旨在促进结核病相关研究工作。考虑到结核分枝杆菌高毒力、复制周期长和需要在生物安全三级实验室中开展研究等特点和难点，该工具为结核病相关研究人员提供了一个强有力的武器。目前这一技术手段的应用已经使我们对结核分枝杆菌-宿主相互作用、小分子-蛋白结合以及抗生素耐药性机制等关键生物过程有了更深入的了解。为了更好地帮助同行了解这一有效的工具，本文综述了结核分枝杆菌功能蛋白组芯片的几种主要应用，期望同行专家能更好地将其应用于结核病相关的基础研究中。

Abstract:

Tuberculosis (TB) is still one of the leading causes of death worldwide. Due to the ever-growing problem of Mycobacterium tuberculosis (Mtb) drug resistance, TB is still not well controlled. There is an urgent need to develop new technology for promoting Mtb basic research, and to reveal new mechanisms, identify new targets, and develop new anti-Mtb drugs. Bore this need in mind, a Mtb functional proteome microarray was constructed to promote basic research. Considering the high virulence of Mtb, long replication cycle, it is very difficult to perform Mtb related studies, thus efficient tool is need. The proteome microarray is a powerful tool for the researchers in the field of TB study. It has already been successfully applied in a wide range of studies. Better understandings of a variety of key biological processes have already been added, for example, Mtb-host interactions, small-molecule-protein binding and mechanism of antibiotic resistance. In order to help readers to better understand this powerful tool, we present several major applications of the Mtb functional proteome microarray in this review, we anticipate researchers will adopt this powerful tool for a variety of TB related studies, thus facilitate both basic and clinical research of TB.