冰岛硫化叶菌遗传操作体系的研究进展

Systems for the genetic manipulation of Sulfolobus islandicus

摘要:

冰岛硫化叶菌是古菌研究中常用的模式菌株，为人们研究古菌复制、细胞周期以及CRISPR-Cas系统等作出了巨大贡献。冰岛硫化叶菌遗传操作体系的建立与完善对古菌学的全面深入研究起至关重要的作用。本文介绍了冰岛硫化叶菌遗传操作体系所使用的质粒载体、筛选标记和转化方法，论述了目前广泛使用的两类冰岛硫化叶菌基因敲除体系。最后提出了现有冰岛硫化叶菌基因操作体系存在的主要问题，并对其发展方向进行了展望。

Abstract:

Sulfolobus islandicus is a commonly used type strain to study replication, cell cycle and CRISPR-Cas system in archaea. For this reason, it is important to establish a genetic manipulation system in Sulfolobus islandicus. Here, we describe the plasmid vector, selection marker and transformation methods, and discuss two currently widely used gene knockout systems. Meanwhile, we also address some major problems in genetic manipulation systems of Sulfolobus islandicus, and indicate development prospects.